Nucleolin Antibody
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL) . It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P19338
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NCL/902
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nucleolin antibody is available for research use only.
Storage Conditions
Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nucleoli
Image Legend
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
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