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MFG-E8 Antibody

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q08431

Host

Mouse

Reactivity

Human

Immunogen

A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM291

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the MFG-E8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MFG-E8 antibody is available for research use only.

Storage Conditions

Store the MFG-E8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cell surface & cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFG-E8 antibody (SPM291)

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