MFG-E8 Antibody
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q08431
Host
Mouse
Reactivity
Human
Immunogen
A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM291
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the MFG-E8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MFG-E8 antibody is available for research use only.
Storage Conditions
Store the MFG-E8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface & cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFG-E8 antibody (SPM291)
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