EpCAM Antibody / Extracellular domain

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

A synthetic peptide (around aa 20-60) from the N-terminus of the human protein was used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EGP40/826

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Right: Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM antibody (EGP40/826) (Green) . F-actin filaments were labeled with DyLight 554 Phalloidin (red) . Left: Negative control. DAPI was used to stain the cell nuclei (blue) .