Fas Antibody / CD95
MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL
UniProt
P25445
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Fas antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
B-R18
Applications
IHC, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Fas antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fas antibody is available for research use only.
Storage Conditions
Store the Fas antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Fas antibody should be determined by the researcher.
Location
Cytoplasmic, membranous
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free Fas Antibody (B-R18) . Confirmation of Integrity and Purity of the Antibody.
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