Uroplakin 3A Antibody

Uroplakin 3A (UPK3A) is a component of the asymmetric unit membrane (AUM) . It is a highly specialized bio-membrane made by terminally differentiated urothelial cells. Uroplakin 3A may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection. Uroplakin 3A has been shown as a helpful marker for the detection of bladder cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide, aa 260-279 (GASESSYTSVNRGPPLDRAE) of human Uroplakin 3A was used as the immunogen for this antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Uroplakin 3A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Uroplakin 3A antibody is available for research use only.

Storage Conditions

Store the Uroplakin 3A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.