CD28 Antibody PE Conjugate

This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CD28 protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CB28

Applications

FACS

Purity

Protein G affinity chromatography

Format

PE Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD28 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Limitations

This CD28 antibody is available for research use only.

Storage Conditions

Store the CD28 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.