CD6 Antibody

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. It shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. CD6 antibody can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

The human rheumatoid synovial T cell line ST-1 was used as the immunogen for this CD6 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

3F7B5

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD6 antibody is available for research use only.

Storage Conditions

Store the CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be tittered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.