Krueppel-like factor 4 Antibody / KLF4

Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O43474

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M1-H389) was used as the immunogen for the Krueppel-like factor 4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Krueppel-like factor 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Krueppel-like factor 4 antibody is available for research use only.

Storage Conditions

After reconstitution, the Krueppel-like factor 4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Krueppel-like factor 4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human skin cancer tissue with Krueppel-like factor 4 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.