PCNA Antibody

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase delta in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry (intracellular) : 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P12004

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids M1-S261) was used as the immunogen for the PCNA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PCNA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PCNA antibody is available for research use only.

Storage Conditions

After reconstitution, the PCNA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with PCNA antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.