PPCS Antibody

Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9HAB8

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids VEIEEKIVDNLQSRHTAFIGDRN were used as the immunogen for the PPCS antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

7G13

Applications

WB, IHC-P, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the PPCS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PPCS antibody is available for research use only.

Storage Conditions

After reconstitution, the PPCS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PPCS antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human tonsil with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.