GAPDH Antibody

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:3000-1:10000, Immunohistochemistry (FFPE) : 1:100-1:250, Immunofluorescence: 1:100-1:250

UniProt

P04406

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human GAPDH was used as the immunogen for the GAPDH antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

BG-7

Type

Recombinant

Applications

IHC-P, WB, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the GAPDH antibody at -20oC.

Limitations

This GAPDH antibody is available for research use only.

Storage Conditions

Store the GAPDH antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the GAPDH antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of 1) rat liver, 2) rat brain, 3) rat RH35, 4) mouse liver, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.