ER alpha Antibody
Estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. [RefSeq]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100, Immunofluorescence: 1:50-1:100
UniProt
P03372
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide specific to human ER alpha / ESR1 was used as the immunogen for the ER alpha antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
IE-5
Type
Recombinant
Applications
IHC-P, WB, IF
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the ER alpha antibody at -20oC.
Limitations
This ER alpha antibody is available for research use only.
Storage Conditions
Store the ER alpha antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the ER alpha antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) MCF7 and 2) T-47D cell lysate with ER alpha antibody. Predicted molecular weight ~66 kDa.
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