HOOK3 Antibody

Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-3ug/10^6 cells, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q86VS8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E14-R198 from the human protein were used as the immunogen for the HOOK3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the HOOK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HOOK3 antibody is available for research use only.

Storage Conditions

After reconstitution, the HOOK3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HOOK3 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) U-87 MG, 3) U-2 OS, 4) K562, 5) Caco-2, 6) A549, 7) rat heart and 8) mouse heart lysate with HOOK3 antibody at 0.5ug/ml. Predicted molecular weight ~83 kDa.