Phospho-IRE1 Antibody (pS724)
IRE1 is a serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) . In unstressed cells, the ER luminal domain is maintained in its inactive monomeric state by binding to the ER chaperone HSPA5/BiP. Accumulation of misfolded protein in the ER causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. [UniProt]
Product Specifications
Specifications
Western blot: 1:500-1:2000
UniProt
O75460
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human IRE1 / ERN1 (surrounding pS724) was used as the immunogen for the phospho-IRE1 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
EGH-5
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the phospho-IRE1 antibody at -20oC.
Limitations
This phospho-IRE1 antibody is available for research use only.
Storage Conditions
Store the phospho-IRE1 antibody at -20°C.
Product Datasheet
https://www.nsjbio.com/tds-pdf/phospho-ire1-antibody-ps724-egh-5-rq4781
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-IRE1 antibody should be determined by the researcher.
CAS Number
9000-83-3
Image Legend
Western blot testing of lysate from 1) untreated and 2) AP-treated human K562 cells with phospho-IRE1 antibody. Predicted molecular weight ~110 kDa.
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