ITGA3 Antibody / Integrin alpha 3

ITGA3 (INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. It is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. This gene is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the a3b1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit. A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL

UniProt

P26006

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Amino acids YSVALHRQTERQQRYLLLAGAPRELAVPDGYTNR were used as the immunogen for the ITGA3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ITGA3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ITGA3 antibody is available for research use only.

Storage Conditions

After reconstitution, the ITGA3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ITGA3 antibody should be determined by the researcher.

Location

Plasma membrane

Image Legend

IHC staining of FFPE human lung cancer with ITGA3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.