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GRP78 Antibody / BiP / HSPA5

Additional name (s) for this target protein: HSPA5; Heat shock 70kDa protein 5; glucose-regulated protein 78

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.3 µg/mL, IF/ICC: 5 µg/mL, ELISA (peptide) LOD: 1:128000

Host

Goat

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KVVEKKTKPYIQVD were used as the immunogen for this GRP78 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF/ICC, ELISA (peptide)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Reconstitution

Aliquot and store the GRP78 antibody at -20oC.

Limitations

This GRP78 antibody is available for research use only.

Storage Conditions

Aliquot and store the GRP78 antibody at -20°C.

Formulation

0.5 mg/mL in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Applications Notes

Optimal dilution of the GRP78 antibody should be determined by the researcher.

Image Legend

Western blot testing of A) HepG2, B) HeLa and C) MCF7 cell lysate with GRP78 antibody at 0.1ug/ml. Predicted molecular weight: ~73 kDa, routinely observed at 70-78 kDa.
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