STAT1 Antibody

Signal transducer and activator of transcription 1 is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells

UniProt

P42224

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Amino acids S2-A230 from the human protein were used as the immunogen for the STAT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the STAT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This STAT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the STAT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the STAT1 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) HeLa, 2) PC-3, 3) Caco-2, 4) A549, 5) K562 and 6) Raji cell lysate with STAT1 antibody at 0.5ug/ml. Predicted molecular weight ~91/84 kDa (alpha/beta) .