AGR2 Antibody / Anterior gradient protein 2

Anterior gradient protein 2 homolog (AGR-2), also known as secreted cement gland protein XAG-2 homolog, is a protein that in humans is encoded by the AGR2 gene. Anterior gradient homolog 2 was originally discovered in Xenopus laevis. In Xenopus AGR2 plays a role in cement gland differentiation, but in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response, cell migration, and cell transformation. However, there have been other observations that AGR2 can repress growth and proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, FACS: 1-3ug/million cells

UniProt

O95994

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids R21-L175 from the human protein were used as the immunogen for the AGR2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the AGR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This AGR2 antibody is available for research use only.

Storage Conditions

After reconstitution, the AGR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the AGR2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human bladder infiltrating urothelial carcinoma with squamous differentiation tissue with AGR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.