CD58 Antibody / LFA3
CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells
Gene ID
965
UniProt
P19256
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids F29-R215 from the human protein were used as the immunogen for the CD58 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
Limitations
This CD58 antibody is available for research use only.
Storage Conditions
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd58-antibody-lfa3-r32395
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CD58 antibody should be determined by the researcher.
Location
Cytoplasmic, cell surface
Image Legend
Western blot testing of human 1) HeLa and 2) K562 cell lysate with CD58 antibody. Predicted molecular weight: 28/40-70 kDa (unmodified/glycosylated) .
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