WASP Antibody

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P42768

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids ADEDEAQAFRALVQEKIQKRNQRQSGDR of human WASP were used as the immunogen for the WASP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the WASP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This WASP antibody is available for research use only.

Storage Conditions

After reconstitution, the WASP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the WASP antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat liver, 2) human placenta, 3) rat spleen, 4) rat pancreas, 5) human HepG2, 6) HeLa, 7) HEPA and 8) 22RV1 lysate with WASP antibody. Expected/observed molecular weight ~53 kDa.