COL2A1 Antibody / Collagen II

Collagen type 2 alpha 1, also known as COL2A1, is the major collagen synthesized by chondrocytes. It is a fibrillar collagen found in cartilage and the vitreous humor of the eye.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

P28481

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse Collagen type 2 (AFAGLGQREKGPDPMQYMRA) was used as the immunogen for this COL2A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the COL2A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This COL2A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the COL2A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the COL2A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of COL2A1 antibody and Lane 1: rat heart; 2: rat brain tissue lysate. Predicted molecular weight ~140 kDa.

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