WISP1 Antibody

WNT1-Inducible Signaling Pathway Protein 1, also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. It is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed (Tanaka et al., 2001) . By use of radiation hybrid mapping panels, Pennica et al. (1998) mapped the gene to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al. (1998) found that two distinct systems demonstrated that WISP1 induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

O95388

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human WNT1-Inducible Signaling Pathway Protein 1 (ADLESYPDFSEIAN) was used as the immunogen for this WISP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the WISP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This WISP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the WISP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the WISP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of rat 1) heart, 2) kidney, 3) lung and human 4) A549, 5) A431 and 6) COLO320 lysate with WISP1 antibody. Expected molecular weight: 40-55 kDa depending on degree of glycosylation.