Annexin VII Antibody

Annexin A7, also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al. (1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al. (2001) concluded that the gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al. (1996) present experimental evidence, based on studies of recombinant human protein and isolated bovine chromaffin cells, that ANXA7 is a Ca (2+) -dependent GTP binding protein. It was active in a chromaffin granule aggregation assays in the presence of Ca (2+) and GTP, and was deactivated upon GTP hydrolysis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P20073

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Annexin VII (TRSEIDLVQIKQMFAQ) was used as the immunogen for this Annexin VII antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Annexin VII antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Annexin VII antibody is available for research use only.

Storage Conditions

After reconstitution, the Annexin VII antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Annexin VII antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with Annexin VII antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.