B Raf Antibody (BRAF)

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf, also known as RAFB1. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts. B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P15056

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 344-362 (QPFRPADEDHRNQFGQRDR) were used as the immunogen for this B Raf antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the B Raf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This B Raf antibody is available for research use only.

Storage Conditions

After reconstitution, the B Raf antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the B Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of B Raf antibody and Lane 1: rat brain; 2: rat testis; 3: rat liver; 4: (h) SW620; 5: (h) COLO320; 6: (r) PC-12 cell lysate. Predicted molecular weight: 85-95 kDa.