Kallikrein 6 Antibody

Kallikrein-related peptidase 6 is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimers disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

Q92876

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human KLK6 (YTNVCRYTNWIQKTIQAK) was used as the immunogen for this Kallikrein 6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Kallikrein 6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Kallikrein 6 antibody is available for research use only.

Storage Conditions

After reconstitution, the Kallikrein 6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Kallikrein 6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Kallikrein 6 antibody and human lysate samples: 1) MCF-7; 2) HeLa; 3) MM231; 4) MM453; 5) A549; 6) SMMC-7721; 7) COLO320; 8) SW620; 9) HT1080. Predicted molecular weight: ~27 kDa.