RFC1 Antibody / Replication factor C subunit 1

Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P35251

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the RFC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RFC1 antibody is available for research use only.

Storage Conditions

After reconstitution, the RFC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the RFC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with RFC1 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.