MDM2 Antibody

MDM2 is an important negative regulator of the p53 tumor suppressor. The protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q00987

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 343-358 (EKAKLENSTQAEEGFD) were used as the immunogen for this MDM2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the MDM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MDM2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MDM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of MDM2 antibody and Lane 1: rat testis; 2: rat brain; 3: rat heart; 4: human SKOV-3; 5: COLO320; 6: HeLa; 7: mouse HEPA; 8: monkey COS7 cell lysate. Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.