CD95 Antibody / Fas

Fas (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

Q63199

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of rat CD95 (YTDRKHYSDKCRRCAFCDEGHGL) was used as the immunogen for this CD95 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the CD95 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD95 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD95 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CD95 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of rat 1) liver, 2) spleen, 3) brain and 4) heart lysate with CD95 antibody at 1ug/ml. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated) .