EAAT2 Antibody

SLC1A2 is also known as EAAT2 or GLT-1. This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Human partial recombinant protein (AA 461-574) was used as the immunogen for this EAAT2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the EAAT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EAAT2 antibody is available for research use only.

Storage Conditions

After reconstitution, the EAAT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the EAAT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE mouse brain tissue with EAAT2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.