Tyrosine Hydroxylase Antibody

Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, TH has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL; formalin or acetone fixed tissues

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Rat tyrosine hydroxylase was used as the immunogen for this Tyrosine Hydroxylase antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

TH-16

Applications

WB, IHC-P, IHC-F

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Tyrosine Hydroxylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Tyrosine Hydroxylase antibody is available for research use only.

Storage Conditions

After reconstitution, the Tyrosine Hydroxylase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Tyrosine Hydroxylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) rat brain, 2) mouse brain and 3) human U-87 MG cell lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight ~59 kDa.