CDC25C Antibody

Cell division cycle (CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34 (cdc2) /cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this CDC25C antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

IMD-25

Applications

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the CDC25C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CDC25C antibody is available for research use only.

Storage Conditions

After reconstitution, the CDC25C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CDC25C antibody may be required due to differences in protocols and secondary/substrate sensitivity.