DEK Antibody
DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
P35659
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 336-364 from the human protein was used as the immunogen for the DEK antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the DEK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DEK antibody is available for research use only.
Storage Conditions
Aliquot the DEK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human 1) U-251 MG and 2) PC-3 cell lysate with DEK antibody. Expected molecular weight: 43-50 kDa.
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