Phospholipase A2 Antibody / PLA2G4A / cPLA2

PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca (2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

P47712

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 513-541 from the human protein was used as the immunogen for the Phospholipase A2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IF, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Phospholipase A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Phospholipase A2 antibody is available for research use only.

Storage Conditions

Aliquot the Phospholipase A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Phospholipase A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.