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Filamin A Antibody / FLNA

Actin-binding protein 280, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

P21333

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1025-1054 from the human protein was used as the immunogen for the Filamin A antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Filamin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Filamin A antibody is available for research use only.

Storage Conditions

Aliquot the Filamin A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Filamin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skeletal muscle tissue with Filamin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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