Pyruvate Kinase PKM Antibody / PKM2

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P14618

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 383-417 from the human protein was used as the immunogen for the Pyruvate Kinase PKM antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Pyruvate Kinase PKM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pyruvate Kinase PKM antibody is available for research use only.

Storage Conditions

Aliquot the Pyruvate Kinase PKM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Pyruvate Kinase PKM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human skin tissue with Pyruvate Kinase PKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.