Brachyury Antibody / Protein T / TBXT

The protein encoded by the TBXT gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25

UniProt

O15178

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 15-43 from the human protein was used as the immunogen for the Brachyury antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Brachyury antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Brachyury antibody is available for research use only.

Storage Conditions

Aliquot the Brachyury antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Brachyury antibody may be required due to differences in protocols and secondary/substrate sensitivity.