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OTU1 Antibody / YOD1 / DUBA-8

Deubiquitinating enzymes (DUBS) are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA-8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. [OMIM].

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

Q5VVQ6

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 319-347 from the human protein was used as the immunogen for the OTU1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the OTU1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This OTU1 antibody is available for research use only.

Storage Conditions

Aliquot the OTU1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the OTU1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western bot testing of two different lots of human K562 cell lysate with OTU1 antibody. Predicted molecular weight ~38 kDa.

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