TSP5 Antibody / COMP

COMP/TSP5 is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P49747

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 314-343 from the human protein was used as the immunogen for the TSP5 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TSP5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TSP5 antibody is available for research use only.

Storage Conditions

Aliquot the TSP5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TSP5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Secreted

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with TSP5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.