CBFB Antibody

Core-binding factor subunit beta is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2) . The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

Q13951

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 61-90 from the human protein was used as the immunogen for the CBFB antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CBFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CBFB antibody is available for research use only.

Storage Conditions

Aliquot the CBFB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CBFB antibody may be required due to differences in protocols and secondary/substrate sensitivity.