TPI1 Antibody / Triosephosphate isomerase

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P60174

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 68-96 from the human protein was used as the immunogen for the TPI1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TPI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TPI1 antibody is available for research use only.

Storage Conditions

Aliquot the TPI1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TPI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with TPI1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.