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CFL1 Antibody / Cofilin 1

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

P23528

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 70-98 from the human protein was used as the immunogen for the CFL1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CFL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CFL1 antibody is available for research use only.

Storage Conditions

Aliquot the CFL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CFL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Flow cytometry testing of human HL60 cells with CFL1 antibody; Blue=isotype control, Green= CFL1 antibody.

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