Vimentin Antibody

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, ICC/IF: 1:800, Immunoprecipitation: suitable

UniProt

P08670

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein was used as the immunogen for this Vimentin antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

4F8-A5-H5

Applications

WB, ICC/IF, IP

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the Vimentin antibody at -20oC.

Limitations

This Vimentin antibody is available for research use only.

Storage Conditions

Store the Vimentin antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.