Anti-SIRT1 Antibody

A NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000

UniProt

Q96EB6

Host

Rabbit

Reactivity

Human

Immunogen

This anti-SIRT1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 587-621 amino acids from the C-terminal region of human SIRT1.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the anti-SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-SIRT1 antibody is available for research use only.

Storage Conditions

Aliquot the anti-SIRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the anti-SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of anti-SIRT1 antibody at 1:2000 dilution. Lane 1: K562; 2: HeLa; 3: A549; 4: human testis lysate; Molecular weight: visualized from 80~120 kDa depending on post-translational modifications