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TLE1 Antibody / Transducin-like enhancer protein 1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Product Specifications

Specifications

Immunofluorescence: 1:25, Western blot: 1:1000

UniProt

Q04724

Host

Rabbit

Reactivity

Human

Immunogen

This TLE1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 187-221 amino acids from the N-terminal region of human TLE1.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TLE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TLE1 antibody is available for research use only.

Storage Conditions

Aliquot the TLE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/tle1-antibody-transducin-like-enhancer-protein-1-f52972

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TLE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

CAS Number

9007-83-4

Image Legend

Fluorescent image of HeLa cells stained with TLE1 antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green) . Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red) .
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