CD14 Antibody

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P08571

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 54-83 from the human protein was used as the immunogen for this CD14 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD14 antibody is available for research use only.

Storage Conditions

Aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, Secreted, Cytoplasmic (Golgi)

Image Legend

Confocal immunofluorescent analysis of CD14 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .