LIN28B Antibody
LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity) . [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
Q6ZN17
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This LIN28B antibody is available for research use only.
Storage Conditions
Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Image Legend
Western blot analysis of LIN28B antibody and 1) HeLa, 2) K562, 3) mouse testis and 4) NCCIT lysate. Predicted molecular weight ~27 kDa.
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