Anti-Src Antibody
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Western blot: 1:100-1000
UniProt
P12931
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This anti-Src antibody was produced from a mouse immunized with a full length recombinant human protein.
Clonality
Monoclonal
Isotype
IgG1
Clone
17AT28
Applications
WB, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-Src antibody is available for research use only.
Storage Conditions
Aliquot the anti-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from (1) HT29 and (2) Jurkat cell line using anti-Src antibody at 1:1000.
Documents
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