5-BrdU

5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

Product Specifications

CAS Number

[59-14-3]

Product Name Alternative

BrdU; 5-Bromo-2'-deoxyuridine; BUdR

UNSPSC

12352005

Hazard Statement

H340, H361

Target

DNA/RNA Synthesis; Nucleoside Antimetabolite/Analog

Type

Oligonucleotides

Related Pathways

Cell Cycle/DNA Damage

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/5-BrdU.html

Purity

99.97

Solubility

DMSO : 250 mg/mL (ultrasonic) |H2O : 2 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O[C@H]1C[C@H](N2C(NC(C(Br)=C2)=O)=O)O[C@@H]1CO

Molecular Formula

C9H11BrN2O5

Molecular Weight

307.10

Precautions

H340, H361

References & Citations

[1]Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10 (8) :804-16.|[2]Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr; Chapter 7:Unit7.31