EcoPlexTM Human Th1/Th2/Th17 11-Plex Panel Kit

Product Specifications

Abbreviation

IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, TNFα, TNFβ

UniProt

TNF-β:P01374 TNF-α:P01375 IL-1β:P01584 IL-2:P60568 IFN-γ:P01579 IL-12p70:P29459&P29460 IL-4:P05112 IL-5:P05113 IL-6:P05231 IL-17A:Q16552 IL-10:P22301

Cross Reactivity

Cross-reactivity of analytes in the Panel: Negligible

Assay Principle

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Assay Performance Time

3 h

Sample Type

Serum, plasma, bodily fluid, cell culture supernatant

Precision

Intra-assay CV<10% Inter-assay CV<20%

Recovery

70%-130%

Sensitivity

TNF-β<5pg/mL TNF-α<1pg/mL IL-1β<5pg/mL IL-2<3pg/mL IFN-γ<3pg/mL IL-12p70<3pg/mL IL-4<1pg/mL IL-5<2pg/mL IL-6<5pg/mL IL-17A<3pg/mL IL-10<2pg/mL

Shipping Conditions

Ice bag

Storage Conditions

-20°C,12 months

Product Datasheet

https://cdn.gentaur.com/products/763/68dd148e061270361e7cb098/datasheet/mph007.pdf

Product MSDS

https://789.bio/eb/L8O8ZS

Species

Human

Recommended Usage

This kit is for detection of IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, TNF-α and TNF-β in human serum, plasma, biological fluid and cell culture supernatant.